13 Ct12, Putative antimicrobial peptide Descriptor Amino acid sequence Ct61, putative antimicrobial

13 Ct12, Putative antimicrobial peptide Descriptor Amino acid sequence Ct61, putative antimicrobial peptide, partial VVINESEAFFGSLLSLGSKLLPSVFKLFQRKKERSIHKRDLEDLYDPYQRNLEMERFLKQLPMY MKGKTLLVVLLVALLIAEELNSFKFGGFLKKMWKSKLAKKLRAKGREMIKDYANRVLEGPQEEAPPAERRR Ct59, Putative antimicrobial peptide, venom MVRKSLIVLLLVSVLVSTFLTTDAPASFDDDFDALDDMNDLDLDDLLDLEPADLVLLDMWANMLENSDFEDDFE anionic peptide ID/Genbank number JZ122341 JZ122311 JZ122338 JZ122337 Ct44, Putative venom metalloprotease-1, partial Ct48, putative venom neuropeptide, partial Ct57, putative metalloprotease, partial Ct51, putative venom metalloprotease, partial Descriptor Amino acid sequence TTMIYLAGIFLFAIASAIPSGRVDVVFPSVETSRSGVKTVKFRALNEEIELKLEPAGEILAKNFAIVNQQPNSIDVEDLKRRIYR DSNNGAALLIDEDGPVTIQGIVNSKFRIEPYESSRTVKDGQVPHKIVEVINDEKSFLNDAVMQIDV GMGKYYCSHATGLAKDADLIMLLVTRELGELKKDGTVKTTLSGIAYVSGVCKQCKKVGVSLDDSDYNERVDT VAHETAHLLGSLHDGEKSSQGGPVGNPGAVDCLQTDGYIMGDRSVNEFKFSECSKKCVKYALSLPEAKCVYESCGF GGNFWKIETRSKMMFGIWILCGTAFFYCHVDAFMEYSNMVPGYNALIRRSQKEPWWKGRMFDNLGYNQAYLVKRNF DEIDNVGFNDFGPGTGRSWLPKRNLELASYNSRRLRL TTMIYLASIFLFAIASAIPSGRVDVVFPSVETSRSGVKTVKFRALNEEIELKLEPAGE ID/Genbank quantity JZ122324 JZ122323 Ct69, putative hypothetical protein, partial Ct70, putative unnamed protein solution, partial Descriptor Amino acid sequence MQLLLLTCLLQLIMVTNKAIASQISQIKHFFHCILVVVCPNSSMYLIMSGS QICLRPRCWIRFPLFRKRNKKGVCSTHENLHDLSSNRREPGWFLS JZ122316 JZ122317 JZ122318 JZ122319 Ct62, putative hypothetical, protein, partial STSRPLKDELASHTDDLAPEPTARAVNSAGQKFNYELFNCNNFNIRYWSWNYRGCWHQTCPPIVTR Ct63, putative phosphomannomutase, partialYFNETEQSRERFVVIYKFMERDSSTICLLDVDGTVTKPRQSITPTMEAFLQKLKDKVVIGLVGGSDISKIAEQMGGMDVIKKYEYVFA ENGLVAYKNGELIAKESIASYLGEEKVQKLINFCLGYMSKLILPVKRGNFIEFRNGLINI Ct64, putative hypothetical protein, partial Ct65, putative hypothetical protein, partial EPSTQSLCECSPFWSSLFSFCVCLSCKICLHSSFFFLNFFIYVCIFLTYFLVYVDLGLYFFYYRLSDQ GGDICHGKYSRRTIIEFQCGSGEGKPMFQFESEDCTYYFIWSTSLACENKKHCIISNGSESYDLTPLSKSTYTVNDLTGKNDLYYLSVCDSContig5053,Antimicrobial peptideContig/Singlet EST (ESTs)SingletSingletContig4963, 4881,Venom componentsContig/Singlet EST (ESTs)SingletContig4832,SingletSingletProteins devoid of defined functionContig/Singlet EST (ESTs)Contig4830-2,Contig5096, 5097, 4957, 5014,4975, 5018, 5073, 4975, 5020, 4931,qSingletSingletSingletProteome Transcriptome of Scorpion C.335599-07-0 Order tecomanusSingletProteome Transcriptome of Scorpion C.Buy5458-56-0 tecomanusSignal peptides are underlined; mature peptides are shown in bold; propeptides proposed are shown in italics.PMID:33501595 doi:ten.1371/journal.pone.0066486.tCt66, putative hypothetical protein, partialCt68, putative hypothetical protein, partialCt67, putative hypothetical protein, partial[53,54], a toxin from C. infamatus infamatus [55] and seven toxins (Css1-7) from C. suffusus suffusus [56]. From the scorpion C. tecomanus five peptides toxic to mammalians were isolated and partially characterized [6?]. Only toxin Clt1 was fully sequenced and shown to include 66 amino acid residues, with 86 sequence identity to toxin II from C. suffusus suffusus toxin II [56]. Clt1 impacts inactivation of Na+channels, when assayed on dorsal root ganglion cells of chicken [7]. It’s clear that the amino acid sequences found by cDNA cloning genes of venom glands of this scorpion (Table 2) plus the mass spectrometry information (Table 1), collectively with the final results of direct amino acid sequence determination (Table S1) of venom components isolated by HPLC (Fig.1), permitted to confirm the information obtained by other authors.